Composite

Part:BBa_K2598049:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-09-27)


CcaSR+cph8+YF1+T7 core


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2031
    Illegal NheI site found at 2054
    Illegal NheI site found at 4604
    Illegal NheI site found at 5250
    Illegal NheI site found at 5273
    Illegal NheI site found at 5293
    Illegal NheI site found at 5316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 7633
    Illegal BamHI site found at 199
    Illegal BamHI site found at 2506
    Illegal XhoI site found at 2958
    Illegal XhoI site found at 5740
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 8299
    Illegal NgoMIV site found at 8371
    Illegal NgoMIV site found at 8461
    Illegal NgoMIV site found at 8479
    Illegal NgoMIV site found at 8971
    Illegal NgoMIV site found at 9264
    Illegal NgoMIV site found at 9358
    Illegal AgeI site found at 3940
    Illegal AgeI site found at 8013
    Illegal AgeI site found at 9139
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9028
    Illegal BsaI.rc site found at 7912


Design Notes

The direction of genes are reversed to avoid leakage.


Source

References

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)